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13. Can I additionally use degenerated base positions (which include a couple of nucleotide, i.e. wobble bases)? Please be aware that the precise ratio of all bases at a degenerated position cannot be predicted. Since a small amount of incorrect bases at any place cannot be seen during sequencing (it is overshadowed by the big variety of right bases at that position), you've got to transform a number with the vector and screen for right clones.

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